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Antibodies bound to the blocking peptide no more bind on the epitope within the target protein. By evaluating the staining in the blocked antibody versus the antibody by itself, you can see which staining is certain.
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This guidebook outlines further attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This guidebook outlines more feasible optimization of mobile and immunoassay parameters to ensure the best possible effects are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope regarded via the antibody.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin spouse and children, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E types a posh with and capabilities as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells progress by S stage.
Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a vital job in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two supplemental splice variants had been described but detailed nucleotide sequence data is not really but readily available.
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